| Specimen ID | State | County | Locality | Year | HUC Number | Drainage Name | Status |
| 616742 | WI | Manitowoc | West Twin River At Shoto Dam [E of Two Rivers] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616744 | WI | Manitowoc | East Twin River at Maplewood Rd. [NW of Two Rivers] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 558525 | WI | Brown | Devils River | 2000 | 04030101 | Manitowoc-Sheboygan | established |
| 558651 | WI | Sheboygan | Mullet River | 2003 | 04030101 | Manitowoc-Sheboygan | established |
| 558713 | WI | Manitowoc | Johnson Creek | 2004 | 04030101 | Manitowoc-Sheboygan | established |
| 616733 | WI | Manitowoc | Manitowoc River at Manitou Park | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616746 | WI | Manitowoc | East Twin River at Mischicot | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616767 | WI | Ozaukee | Sauk Creek | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616770 | WI | Manitowoc | East Twin River at Hwy VV | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616781 | WI | Manitowoc | Little Manitowoc River at Chiropractor | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616782 | WI | Manitowoc | Silver Creek at Silver Creek Road | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 1427320 | WI | Calumet | Pine Creek | 2014 | 04030101 | Manitowoc-Sheboygan | established |
| 1427514 | WI | Sheboygan | Sheboygan River | 2015 | 04030101 | Manitowoc-Sheboygan | established |
| 1423592 | WI | Manitowoc | Harpt Lake | 2014 | 04030101 | Manitowoc-Sheboygan | established |
| 1424251 | WI | Calumet | Killsnake River W of Killsnake Rd and CTH BB | 2014 | 04030101 | Manitowoc-Sheboygan | established |
| 1426467 | WI | Manitowoc | Mud Creek | 2005 | 04030101 | Manitowoc-Sheboygan | established |
| 616783 | WI | Manitowoc | East Twin River at Sturm Road | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616784 | WI | Manitowoc | Manitowoc River at County R Park | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616785 | WI | Manitowoc | West Twin River at Dam (upstream) | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 850889 | WI | Manitowoc | Manitowoc River, Franklin Street, Lake Michigan trib | 2005 | 04030101 | Manitowoc-Sheboygan | established |
| 850890 | WI | Ozaukee | Sauk Creek, Hwy 32 and 22, Lake Michigan trib | 2005 | 04030101 | Manitowoc-Sheboygan | established |
| 1427026 | WI | Sheboygan | Pigeon River - Maywood Environmental Park | 2013 | 04030101 | Manitowoc-Sheboygan | established |
| 1427369 | WI | Calumet | S Br Manitowic R - at Chilton Library | 2016 | 04030101 | Manitowoc-Sheboygan | established |
| 1427609 | WI | Manitowoc | Silver Mw #99 - Across From 3224 Hwy Cr | 2015 | 04030101 | Manitowoc-Sheboygan | established |
| 1422782 | WI | Manitowoc | Meeme River at Wilmas Rd | 2009 | 04030101 | Manitowoc-Sheboygan | established |
| 1423068 | WI | Kewaunee | Tisch Mills Creek at Cty Hwy BB | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1423305 | WI | Brown | Twin Hill Creek DS CTH NN | 2014 | 04030101 | Manitowoc-Sheboygan | established |
| 1424110 | WI | Calumet | Jordan Creek | 2017 | 04030101 | Manitowoc-Sheboygan | established |
| 1425240 | WI | Manitowoc | Cedar Lake | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1425397 | WI | Sheboygan | Little Elkhart Lake | 2016 | 04030101 | Manitowoc-Sheboygan | established |
| 1425484 | WI | Manitowoc | Branch River | 2012 | 04030101 | Manitowoc-Sheboygan | established |
| 1426095 | WI | Manitowoc | DELETE - Little Manitowoc River - Reed Ave | 2015 | 04030101 | Manitowoc-Sheboygan | established |
| 1426440 | WI | Manitowoc | Mud Creek | 2005 | 04030101 | Manitowoc-Sheboygan | established |
| 1423776 | WI | Manitowoc | Valders Creek at Washington St | 2015 | 04030101 | Manitowoc-Sheboygan | established |
| 1424138 | WI | Manitowoc | Valder Creek - N of Washington St | 2015 | 04030101 | Manitowoc-Sheboygan | established |