| Specimen ID | State | County | Locality | Year | HUC Number | Drainage Name | Status |
| 166779 | WI | Sheboygan | Sheboygan River | 2006 | 04030101 | Manitowoc-Sheboygan | established |
| 268529 | WI | Sheboygan | Sheboygan River at Esslinger Park off Taylor Road [in Sheboygan] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 268531 | WI | Sheboygan | Sheboygan River at Blackwolf Run Golf Course [in Sheboygan] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 268532 | WI | Sheboygan | Sheboygan River in downtown Sheboygan (near mouth) | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 268533 | WI | Sheboygan | Pidgeon River at Lakeshore Drive Bridge [in Sheboygan] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 268534 | WI | Sheboygan | Pidgeon River at JayCee Park (Hwy 42 Bridge) [in Sheboygan] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 268536 | WI | Ozaukee | Lake Michigan - outer breakwall at Port Washington Harbor | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 268537 | WI | Manitowoc | Silver Creek at Mouth (in county park south of Manitowoc) | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 268538 | WI | Manitowoc | Little Manitowoc River at mouth [in Manitowoc] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 268546 | WI | Manitowoc | Twin Rivers [East Twin and West Twin rivers] at mouths [confluence in Two Rivers] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 268562 | WI | Manitowoc | West Twin River At Shoto Dam [E of Two Rivers] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 268564 | WI | Manitowoc | East Twin River at Mischicot Dam [in Mischicot] | 2009 | 04030101 | Manitowoc-Sheboygan | established |
| 268565 | WI | Manitowoc | East Twin River at Sturm Road [NW of Two Rivers] | 2009 | 04030101 | Manitowoc-Sheboygan | established |
| 268563 | WI | Manitowoc | East Twin River at Maplewood Rd. [NW of Two Rivers] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616745 | WI | Manitowoc | East Twin River at Mischicot | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616732 | WI | Manitowoc | Manitowoc River at Manitou Park | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 793337 | WI | Sheboygan | [No specific locality provided] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 793338 | WI | Sheboygan | [No specific locality provided] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 793339 | WI | Sheboygan | [No specific locality provided] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 793340 | WI | Sheboygan | [No specific locality provided] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 793341 | WI | Sheboygan | [No specific locality provided] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 793398 | WI | Manitowoc | [No specific locality provided] | 2008 | 04030101 | Manitowoc-Sheboygan | established |
| 793399 | WI | Manitowoc | [No specific locality provided] | 2008 | 04030101 | Manitowoc-Sheboygan | established |
| 793426 | WI | Sheboygan | Sheboygan River | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 793432 | WI | Sheboygan | Sheboygan River | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 268566 | WI | Manitowoc | Manitowoc River at County R Park [in Manitowoc] | 2009 | 04030101 | Manitowoc-Sheboygan | established |
| 616734 | WI | Manitowoc | Manitowoc River at mouth | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 616728 | WI | Sheboygan | Sheboygan River | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 644327 | WI | Manitowoc | East Twin River dam in city of Mishicot, Wisconsin | 2014 | 04030101 | Manitowoc-Sheboygan | established |
| 793371 | WI | Manitowoc | [No specific locality provided] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 793373 | WI | Manitowoc | East Twin River | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 793374 | WI | Manitowoc | [No specific locality provided] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 793433 | WI | Sheboygan | Sheboygan River | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652935 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652936 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652937 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652938 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652939 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652940 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652941 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652942 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652943 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652954 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652955 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652956 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652957 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652958 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652959 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652960 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652961 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652962 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652973 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2014 | 04030101 | Manitowoc-Sheboygan | established |
| 1652974 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652931 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2009 | 04030101 | Manitowoc-Sheboygan | established |
| 1652932 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2009 | 04030101 | Manitowoc-Sheboygan | established |
| 1652933 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2009 | 04030101 | Manitowoc-Sheboygan | established |
| 1652934 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652944 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652945 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652946 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652947 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652948 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652949 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2010 | 04030101 | Manitowoc-Sheboygan | established |
| 1652950 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652951 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652952 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1427516 | WI | Sheboygan | Sheboygan River at 8th Street | 2015 | 04030101 | Manitowoc-Sheboygan | established |
| 793427 | WI | Sheboygan | Sheboygan River | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 793428 | WI | Sheboygan | Sheboygan River | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 793429 | WI | Sheboygan | Unnamed Single-line Stream T15n-r23e-s28 | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 793431 | WI | Sheboygan | Sheboygan River | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1425373 | WI | Manitowoc | Little Manitowoc River | 2015 | 04030101 | Manitowoc-Sheboygan | established |
| 1551661 | WI | Ozaukee | [No locality description provided] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 1553047 | WI | Manitowoc | [No locality description provided] | 2008 | 04030101 | Manitowoc-Sheboygan | established |
| 588183 | WI | Manitowoc | [No locality description provided by MARIS] | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 793441 | WI | Sheboygan | Pigeon River | 2012 | 04030101 | Manitowoc-Sheboygan | established |
| 793442 | WI | Sheboygan | Pigeon River | 2012 | 04030101 | Manitowoc-Sheboygan | established |
| 1710521 | WI | Manitowoc | East Twin River at Mischicot | 2007 | 04030101 | Manitowoc-Sheboygan | |
| 1710525 | WI | Sheboygan | Sheboygan River at Sheboygan Falls | 2007 | 04030101 | Manitowoc-Sheboygan | |
| 1710526 | WI | Manitowoc | West Twin River at Dam (Upstream) | 2007 | 04030101 | Manitowoc-Sheboygan | |
| 1710530 | WI | Manitowoc | Manitowoc River at County R Park | 2007 | 04030101 | Manitowoc-Sheboygan | |
| 1710532 | WI | Sheboygan | Pigeon River at County Road Y | 2007 | 04030101 | Manitowoc-Sheboygan | |
| 1710539 | WI | Manitowoc | Little Manitowoc River at Chiropractor | 2007 | 04030101 | Manitowoc-Sheboygan | |
| 1710540 | WI | Manitowoc | Silver Creek at Silver Creek Road | 2007 | 04030101 | Manitowoc-Sheboygan | |
| 1653495 | WI | Sheboygan | Sheboygan River, Taylor Bridge down from Esslinger Park | 2007 | 04030101 | Manitowoc-Sheboygan | established |
| 1652953 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652963 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2011 | 04030101 | Manitowoc-Sheboygan | established |
| 1652964 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2012 | 04030101 | Manitowoc-Sheboygan | established |
| 1652965 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2012 | 04030101 | Manitowoc-Sheboygan | established |
| 1652966 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2012 | 04030101 | Manitowoc-Sheboygan | established |
| 1652967 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2012 | 04030101 | Manitowoc-Sheboygan | established |
| 1652968 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2012 | 04030101 | Manitowoc-Sheboygan | established |
| 1652969 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2013 | 04030101 | Manitowoc-Sheboygan | established |
| 1652970 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2013 | 04030101 | Manitowoc-Sheboygan | established |
| 1652971 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2013 | 04030101 | Manitowoc-Sheboygan | established |
| 1652972 | WI | Manitowoc | East Twin River, at sea lamprey barrier in Mishicot | 2009 | 04030101 | Manitowoc-Sheboygan | established |
| 1737248 | WI | Ozaukee | [No locality description provided] | 2016 | 04030101 | Manitowoc-Sheboygan | |
| 1738199 | WI | Sheboygan | [No locality description provided] | 2022 | 04030101 | Manitowoc-Sheboygan | |
| 1888150 | WI | Sheboygan | [No locality description provided] | 2024 | 04030101 | Manitowoc-Sheboygan | |
| 1888273 | WI | Sheboygan | [No locality description provided] | 2024 | 04030101 | Manitowoc-Sheboygan | |
| 1889576 | WI | Manitowoc | [No locality description provided] | 2025 | 04030101 | Manitowoc-Sheboygan | |