| Specimen ID | State | County | Locality | Year | HUC Number | Drainage Name | Status |
| 1715801 | OH | Sandusky | Sandusky River, backwater area approx. 2 miles downstream of I-90/Ohio Turnpike | 2023 | 04100011 | Sandusky | |
| 1920848 | OH | Sandusky | Lake Erie, at Sandusky Bay, OH | 2024 | 04100011 | Sandusky | |
| 1920852 | OH | Sandusky | Lake Erie, at Sandusky Bay, OH | 2024 | 04100011 | Sandusky | |
| 1920862 | OH | Sandusky | Lake Erie, at Sandusky Bay, OH | 2024 | 04100011 | Sandusky | |
| 1923944 | OH | Sandusky | Lake Erie, at Sandusky Bay, OH | 2025 | 04100011 | Sandusky | |
| 1923969 | OH | Sandusky | Lake Erie, at Sandusky Bay, OH | 2025 | 04100011 | Sandusky | |
Citation for this information:
U.S. Geological Survey, 2026,
Gambusia affinis: U.S. Geological Survey, Nonindigenous Aquatic Species Database, Gainesville, FL, https://nas.er.usgs.gov, Access Date: 4/16/2026