| Specimen ID | State | County | Locality | Year | HUC Number | Drainage Name | Status |
| 40076 | OH | Lucas | Maumee River near Lake Erie | 1980 | 04100009 | Lower Maumee | established |
| 39919 | OH | Lucas | Oak Openings near Toledo | 1981 | 04100009 | Lower Maumee | established |
| 40049 | OH | Lucas | Oak Openings near Toledo | 1947 | 04100009 | Lower Maumee | established |
| 748105 | OH | Cuyahoga | Rocky River | 2009 | 04110001 | Black-Rocky | established |
| 1715801 | OH | Sandusky | Sandusky River, backwater area approx. 2 miles downstream of I-90/Ohio Turnpike | 2023 | 04100011 | Sandusky | |
| 1758094 | OH | Ottawa | [No locality description provided] | 2024 | 04100010 | Cedar-Portage | |
| 1758095 | OH | Wood | [No locality description provided] | 2024 | 04100010 | Cedar-Portage | |
| 1758096 | OH | Wood | [No locality description provided] | 2024 | 04100010 | Cedar-Portage | |
| 1758097 | OH | Wood | [No locality description provided] | 2024 | 04100010 | Cedar-Portage | |
Citation for this information:
U.S. Geological Survey, 2025,
Gambusia affinis: U.S. Geological Survey, Nonindigenous Aquatic Species Database, Gainesville, FL, https://nas.er.usgs.gov, Access Date: 12/21/2025