Species Profile - Ulva (Enteromorpha) prolifera

Ulva (Enteromorpha) prolifera O.F. Müller, 1778

Common Name: Green alga, grass kelp, sea lettuce

Synonyms and Other Names:

Enteromorpha compressa var. prolifera, Enteromorpha compressa var. trichodes, Enteromorpha polyclados, Enteromopha prolifera, Enteromorpha salina, Enteromorpha salina var. polyclados, Ulva compressa var. prolifera, Ulva enteromorpha f. prolifera

Identification: This green, filamentous, branched, monosiphonous, and benthic macroalga has hollow thalli with longitudinal rows of quadrangular to polygonal cells. Cells contain starch grains, large parietal chloroplasts, and vacuoles that reach up to 40% of cell volume (Blomster et al. 1998, Hadi et al. 1989, Hayden et al. 2003, Holt 1980b, Young et al. 1984). Fronds of U. prolifera are known to reach 18 cm–1 m long. Cells are around 8–18 μm long (Hadi et al. 1989, Ohno et al. 1999, Ohno and Takahasi 1988, Young et al. 1984).

Size: fronds to 1m

Native Range: Unclear. Ulva prolifera is typically a marine species, known in North America from the coasts, inland salt springs, and western salt lakes (Catling and McKay 1980, Mills et al. 1993). It is present in various waters, generally brackish or salty, in Asia, Europe, and Central America (Doroftei et al. 2001, Gazale and Morucci 1990, 1991, Grintal 1974, Hadi et al. 1989, Han et al. 2002, Holt 1980a, Huh et al. 2004, Kukk 1978, Markham and Munda 1977, McClanahan et al. 2005, Pacheco-Ruiz and Zertuche-Gonzalez 1996, Palomo et al. 2004, Partaly 1978, Polderman 1975, Riouall 1976, Saifullah and Nizamuddin 1977 Schories et al. 1997, Storelli et al. 2001, Wennberg 1992, Yagci 2006, Yamaguchi et al. 2006).


This map only depicts Great Lakes introductions. Click here for Great Lakes region collection information

Great Lakes Nonindigenous Occurrences: Ulva prolifera was first recorded in 1979 near the Windsor Salt Factory in Ontario, within the Lake St. Clair drainage (Catling and McKay 1980, Mills et al. 1993).

Table 1. Great Lakes region nonindigenous occurrences, the earliest and latest observations in each state/province, and the tally and names of HUCs with observations†. Names and dates are hyperlinked to their relevant specimen records. The list of references for all nonindigenous occurrences of Ulva (Enteromorpha) prolifera are found here.

State/Province	First Observed	Last Observed	Total HUCs with observations†	HUCs with observations†
MI	1979	1979	1	Lake St. Clair

Table last updated 5/22/2022

† Populations may not be currently present.

Ecology: This edible crop species comprises different strains that are known to survive, grow, and reproduce in very low salinity (0.1–1‰), brackish, salty, or briny waters. Ulva prolifera has been recorded from a wide range of water temperatures, but many strains prefer temperatures around 15–25°C and reach an upper critical limit at 30–34°C. Ulva prolifera grows well in eutrophic conditions, in which it can become a dominant benthic species. It can sometimes be limited by phosphorus (Dan et al. 2002, Koeman and Van Den Hoek 1982, McClanahan et al. 2005, Murase et al. 2005, O’Brien and Wheeler 1987, Ohno et al. 1999, Ohno and Miyanoue 1980, Ohno and Takahasi 1988, Palomo et al. 2004, Soe-Htun et al. 1986, Yagci 2006).

Ulva prolifera exhibits different reproductive strategies. Some plants are sexual and produce biflagellate anisogametes and quadriflagellate meiospores, while others are asexual and can either produce biflagellate or quadriflagellate negatively phototactic zooids. Plants of different sexuality may not be morphologically distinct, and some populations comprise plants of mixed life histories. Zygotes can remain dormant for a year or more in female filaments of sexual plants. Different populations of U. prolifera frequently exhibit high genetic diversity because plants can reproduce sexually, are highly fecund, are perennials (allowing for the accumulation of diverse traits), and undergo a colonization process involving highly diverse propagules on which various selective forces act (Hirakoka et al. 2003, Huh et al. 2004).

Ulva prolifera is known to host gammarid amphipod species in a brackish lake in Japan (Yamaguchi et al. 2006). In European coastal waters, it is preferred over its congener U. intestinalis as a surface for benthic diatom colonization because it is monosiphonous and not broad or flattened (Holt 1980b).

Means of Introduction: Unknown, although U. prolifera was very likely introduced from the Atlantic coast of North America to the Great Lakes basin.

Status: The last record was from 1979 and it is unknown what the current status of the population is.

Great Lakes Impacts:

There is little or no evidence to support that Ulva prolifera has significant environmental impacts in the Great Lakes.
Realized:
While blooms of U. prolifera have had significant negative environmental and socio-economic impacts in Asia (Xu et al. 2012), these impacts have not been realized in the Great Lakes. Moreover, the persistence of a population in the Great Lakes region is still uncertain.

Potential:
Negative effects have not been realized in the Great Lakes because of the small and uncertain nature of the U. prolifera population (Mills et al. 1993).

However, in recent years, large blooms (over 1 million tons) of U. prolifera on the coastline of Asia have lead to declines in seagrass beds due to shading, disruption of feeding by wading birds, and an overall loss of algal biodiversity (Xu et al. 2012). In those “green tides,” Ulva prolifera released allelochemicals that inhibited growth and photosynthesis in native competitors (Xu et al. 2012). The massive blooms covered large extents of the sea bottom (13,000-30,000 km²) and decoupled biogeochemical cycles between the sediments and the water column. These chemical changes exposed native flora and fauna to oxygen deficiency and anoxia (Xu et al. 2012).

Ulva prolifera mats that formed on intertidal sandflats in Scotland were also found to significantly decrease the macrofaunal diversity. These negative impacts were particularly on species that use planktonic larval recruitment (Bloam et al. 2000). In intertidal flats of the Wadden Sea, dense mats of U. prolifera and other Ulva spp. have been associated with fewer occurrences of brown and red algae (Schories et al. 1997).

Domestically, U. prolifera, among other species, has been responsible for green tides in waters off the coast of Washington (Waaland and Hayden 1998).

There is little or no evidence to support that Ulva prolifera has significant socio-economic impacts in the Great Lakes.
Potential:
China has been experiencing an increase in U. prolifera-dominated green tides since 2007, with a notable event just prior to the 2008 Beijing Olympics. There were significant impacts on the tourism industry as well as on aquaculture. The cost for emergency mitigation action in China during the 2008 bloom was estimated to cost around 200 million Euro. According to Nai-hao et al. (2011) green tides are responsible for aquaculture losses of approximately 86 million Euro annually.

In Spanish waters, U. prolifera is one of the species responsible for fouling intertidal oyster culture systems, although this problem can be partly controlled by snail grazing (Cigarria et al. 1998).

There is little or no evidence to support that Ulva prolifera has significant beneficial effects in the Great Lakes.
Potential:
Zhuang et al. (2012) proposed the use of U. prolifera as green feedstock, biofuel substitute, and chemical production. Ulva prolifera is edible and is considered an economically viable food option in Japan and China (Dan et al. 2002).

Management:

Regulations (pertaining to the Great Lakes region)
There are no known regulations for this species.

Note: Check federal, state/provincial, and local regulations for the most up-to-date information.

Control
Biological
There are no known biological control methods for this species.

Physical
Ulva prolifera can be physically harvested from the water and beaches. However, depending on the size of the bloom this may not be an economically viable option (Nai-hoa et al. 2011).

Chemical
Ulva prolifera blooms occur in eutrophic marine waters. As a result, the reduction of pollution and nutrient run-off could decrease the viable habitat for U. prolifera.

Note: Check state/provincial and local regulations for the most up-to-date information regarding permits for control methods. Follow all label instructions.

Remarks: Ulva was described by Linneaus to consist of green seaweeds with distromatic blades. Link, later removed some species from Ulva and moved species that had a distinct tubular form to Enteromorpha. After conducting a phylogenetic DNA analysis, Hayden et al. (2003) found that the separation of the two genera was artificial and that species in Enteromorpha should be returned to the original Ulva classification. While ITIS lists U. flexuosa under the genus Enteromorpha, Enteromorpha species are now recognized as belonging to the genus Ulva.

References: (click for full references)

Blomster, J., C.A. Maggs, and M.J. Stanhope. 1998. Molecular and morphological analysis of Enteromorpha intestinalis and E. compressa (Chlorophyta) in the British Isles. Journal of Phycology 34: 319-340.

Bolam, S.G., T.F. Fernandes, P. Read, and D. Raffaelli. 2000. Effects of macroalgal mats on intertidal sandflats: an experimental study. Journal of Experimental Marine Biology and Ecology 249(1): 123-137.

Catling, P.M., and W.G. McKay. 1980. Halophytic plants in southern Ontario. Canadian Field Naturalist 94(3): 248-258.

Cigarria, J., J. Fernandez, and L.P. Magadan. 1998. Feasibility of biological control of algal fouling in intertidal oyster culture using periwinkles. Journal of Shellfish Research 17(4): 1167-1169.

Dan, A., M. Hiraoka, M. Ohno, and A.T. Critchley. 2002. Observations on the effect of salinity and photon fluence rate on the induction of sporulation and rhizoid formation in the green alga Enteromorpha prolifera (Muller) J. Agardh (Chlorophyta, Ulvales). Fisheries Science (Tokyo) 68(6): 1182-1188.

Doroftei, E., D. Sava, A. Brezeanu, and M. Arcus. 2001. Macrophytic green algae along the Romanian Black Sea Coast: actual state, ultrastructure and in vitro cultivation. Phycologia 40(Supplement 4): 69.

Gazale, V., and C. Morucci. 1990/1991. Aspects of benthic vegetal population of Porto Pozzo Lagoon, Sardinia. Bollettino della Societa’ Sarda di Scienze Naturali 28: 179-190.

Grintal, A.R. 1974. Marine algae of the southwestern part of the Kara Sea, USSR. Novosti Sistematiki Nizshikh Rastenii 11: 112-116.

Hadi, R., A.M. Hadi, K.M. Bahram, and A.A.S. Hassan. 1989. The new recorded species of Enteromorpha in Baghdad area, Iraq. Bulletin of the Iraq Natural History Museum 8(2): 163-172.

Han, L.J., Z. Fan, and X.J. Yan. 2002. The betaines from Chinese seaweeds. Chinese Journal of Oceanology and Limnology 20(1): 97-100.

Hayden, H.S., J. Blomster, C.A. Maggs, P.C. Silva, M.J. Stanhope, and J.R. Waaland. 2003. Linnaeus was right all along: Ulva and Enteromorpha are not distinct genera. European Journal of Phycology 38: 277-294.

Hiraoka, M., A. Dan, S. Shimada, M. Hagihira, M. Migita, and M. Ohno. 2003. Different life histories of Enteromorpha prolifera (Ulvales, Chlorophyta) from four rivers on Shikoku Island, Japan. Phycologia 42(3): 275-284.

Holt, G. 1980a. A study of the flora of the land locked fjord Onna near Langangen, Telemark, southeastern Norway. Blyttia 38(2): 73-80.

Holt, G. 1980b. Benthic diatoms on green algae in Norway and Faeroe Isles, Scotland, UK. Blyttia 38(1): 9-18.

Huh, M.K., H.Y. Lee, B.K. Lee, and J.S. Choi. 2004. Genetic diversity and relationships between wild and cultivated populations of the sea lettuce, Enteromorpha prolifera, in Korea revealed by RAPID markers. Protistology 3(4): 243-250.

Koeman, R.P.T., and C. van den Hoek. 1982. The taxonomy of Enteromorpha Chlorophyceae in the Netherlands. 2. The section Proliferae. Cryptogamie Algologie 3(1): 37-70.

Kukk, K.H.A. 1978. Benthic vegetation of coastal waters of the southern shore of the Gulf of Finland. Botanicheskii Zhurnal (St. Petersburg) 63(6): 844-852.

Markham, J.W., and I.M. Munda. 1977. A find of Zostera marina at Helgoland Germany. Aquatic Botany 3(1): 91-93.

McClanahan, T.R., R.S. Steneck, D. Pietri, B. Cokos, and S. Jones. 2005. Interaction between inorganic nutrients and organic matter in controlling coral reef communities in Glovers Reef, Belize. Marine Pollution Bulletin 50(5): 566-575.

Mills, E.L., J.H. Leach, J.T. Carlton, and C.L. Secor. 1993. Exotic species in the Great Lakes: a history of biotic crises and anthropogenic introductions. Journal of Great Lakes Research 19(1): 1-54.

Murase, N., H. Kito, Y. Fukumoto, M. Minami, H. Haraguchi, and A. Hirosawa. 2006. Growth, photosynthesis and respiration characteristics in a high temperature region of Enteromorpha prolifera. Journal of National Fisheries University 53(3): 131-136.

Nai-hao, Y., X.W. Zhang, Y.Z. Mao, C.W. Liang, D. Xu, J. Zou, Z.M. Zhuang, and Q.Y. Wang. ‘Green tides’ are overwhelming the coastline of our blue planet: taking the world’s largest example. Ecological Research 26(3): 477-485.

O’Brien, M.C., and P.A. Wheeler. 1987. Short term uptake of nutrients by Enteromorpha prolifera Chlorophyceae. Journal of Phycology 23(4): 547-556.

Ohno, M., and I. Takahashi. 1988. The horizontal and vertical distribution of the food alga Enteromorpha prolifera in Shimanto River, southern Japan. Reports of the Usa Marine Biological Institute, Kochi University 10: 45-54.

Ohno, S., and K. Miyanoue. 1980. Ecology of the food alga Enteromorpha prolifera. Reports of the Usa Marine Biological Institute, Kochi University 2: 11-18.

Ohno, M., S. Mizutani, S. Taino, and I. Takahashi. 1999. Ecology of the edible green alga Enteromorpha prolifera in Shimanto River, southern Japan. Bulletin of the Marine Sciences and Fisheries Kochi University 19: 27-35.

Pacheco-Ruiz, I., and J.A. Zertuche-Gonzalez. 1996. Green algae (Chlorophyta) from Bahia de los Angeles, Gulf of California, Mexico. Botanica Marina 39(5): 431-433.

Palomo, L., V. Clavero, J.J. Izquierdo, A. Aviles, J. Becerra, and F.Z. Niell. 2004. Influence of macrophytes on sediment phosphorus accumulation in a eutrophic estuary (Palmones River, Southern Spain). Aquatic Botany 80(2): 103-113.

Partaly, E.M. 1978. Some ecological features of the Zoothamnium hentscheli (Peritrichia, Infusoria) a mass species of the micro overgrowth in the Sea of Azov. Zhurnal Obschchei Biologii 39(2):248-253.

Polderman, P.J.G. 1975. The algal communities of the northeastern part of the salt marsh De Mok on Texel, the Netherlands. Acta Botanica Neerlandica 24(5-6): 361-378.

Riouall, R. 1976. Quantitative study of macrophytic algae of loose substratum from Prevost Pond, Herault, France. Naturalia Monspeliensia 26: 73-94.

Saifullah, S.M., and M. Nizamuddin. 1977. Studies of the marine algae from Pakistan (Ulvales). Botanica Marina 20(8): 521-536.

Schories, D., A. Albrecht, and H. Lotze. 1997. Historical changes and inventory of macroalgae from Koenigshafen Bay in the northern Wadden Sea. Helgolaender Meeresuntersuchungen 51(3): 321-341.

Shoe-Htun, U., M. Ohno, and S. Mizuta. 1986. Effects of salinity and temperature on the growth of the green alga Enteromorpha prolifera in culture. Reports of the Usa Marine Biological Institute Kochi University 8: 9-14.

Storelli, M.M., A. Storelli, and G.O. Marcotrigiano. 2001. Heavy metals in the aquatic environment of the southern Adriatic Sea, Italy: macroalgae, sediments and benthic species. Environment International 26(7-8): 505-509.

Waaland, J.R., and H.S. Hayden. 1998. Green tide algae of the Padilla Bay Estuary, Washington. Journal of Phycology 34(Supplement 3): 62.

Wennberg, T. 1992. Colonization and succession of macroalgae on a breakwater in Laholm Bay, a eutrophicated brackish water area, SW Sweden. Acta Phytogeographica Suecica 78: 65-77.

Yagci, M.A. 2006. The macrobenthic algae of Beymelek Lagoon (Antalya-Turkey). Turkish Journal of Fisheries & Aquatic Sciences 6(2): 137-147.

Yamaguchi, T., H. Ariyama, T. Mukai, and K. Yamaguchi. 2006. Gammaridian fauna on a red alga Gracilaria asiatica and a green alga Enteromorpha prolifera in a brackish lake, Nakaumi, western Honshu, Japan. Japanese Journal of Limnology 67(3): 223-229.

Young, A.J., J.C. Collins, and G. Russell. 1984. Ultrastructural characterization of taxa in the genus Enteromorpha. Pp. 343-353 in D. E. G. Irvine and D. M. John (eds.) Systematics Association Special Volume No. 27. Systematics of the Green Algae. Academic Press Inc., Orlando, Florida. 449 pp.

Xu, D., Z. Gao, X. Zhang, X. Fan, Y. Wang, D. Li, W. Wang, Z. Zhuang, and N. Ye. Allelopathic interactions between the opportunisitic species Ulva prolifera and the native macroalga Gracilaria lichvoides. PLoS ONE 7(4): 36-48.

Zhuang, T., J. Guo, L. Chen, D. Li, J. Liu, and N. Ye. Microwave-assisted direct liquefaction of Ulva prolifera for bio-oil production by acid catalysis. Bioresource Technology 116: 133-139.

Other Resources:

Great Lakes Waterlife

Author: Kipp, R.M., M. McCarthy, and A. Fusaro

Contributing Agencies:

Revision Date: 5/18/2021

Citation for this information:
Kipp, R.M., M. McCarthy, and A. Fusaro, 2022, Ulva (Enteromorpha) prolifera O.F. Müller, 1778: U.S. Geological Survey, Nonindigenous Aquatic Species Database, Gainesville, FL, and NOAA Great Lakes Aquatic Nonindigenous Species Information System, Ann Arbor, MI, https://nas.er.usgs.gov/queries/GreatLakes/FactSheet.aspx?Species_ID=2714, Revision Date: 5/18/2021, Access Date: 5/22/2022

This information is preliminary or provisional and is subject to revision. It is being provided to meet the need for timely best science. The information has not received final approval by the U.S. Geological Survey (USGS) and is provided on the condition that neither the USGS nor the U.S. Government shall be held liable for any damages resulting from the authorized or unauthorized use of the information.